Elizabeth Shepherd appeared as both Lady Rowena and Ligeia in Roger Corman's The Tomb of Ligeia (1965), as well as Damien: Omen II as the ill-fated reporter Joan Hart, The Kidnapping of the President, Deadly Companion and Amelia. Her TV work includes the cult series The Corridor People (1966), the 1978 miniseries The Bastard, and The Cleopatras, a BBC historical drama.
Shepherd began acting in television series in 1959. In 1960, she appeared in an adaptation of A. J. Cronin's novel, The Citadel. She was the original choice to play Emma Peel in the 1960s television series The Avengers. In 1970 she appeared on Broadway in Barry England's Conduct Unbecoming, a story of the British Army in Kipling's India, as Mrs. Hasseltine. She was praised for her performance in Time magazine. Shepherd was pictured in Time along with her co-stars, the pop singers Jeremy Clyde and Paul Jones, who began their roles as British subalterns in London during 1969.